Dr. Ryo Akashi
My Social Links

Dr. Ryo Akashi

Professor
University of Miyazaki, Japan

Highest Degree
Ph.D. in Plant Production from Kagoshima University, Japan

Share this Profile

Area of Interest:

Plant and Soil Sciences
100%
Agricultural Sciences
62%
Genetic Diversity
90%
Molecular Breeding
75%
Plant Production
55%

Research Publications in Numbers

Books
0
Chapters
0
Articles
0
Abstracts
0

Selected Publications

  1. Ishigaki, G., P. Nitthaisong, Y.S. Prasojo, I. Kobayashi, K. Fukuyama, M.M. Rahman and R. Akashi, 2018. Short-term grazing behavior of cattle under indoor housing for a new-bred tetraploid ruzigrass (Brachiaria ruziziensis Germain et Everard). Asian-Australas. J. Anim. Sci., 31: 748-754.
    CrossRef  |  PubMed  |  Direct Link  |  
  2. Hashiguchi, M., H. Tanaka, M. Muguerza, R. Akashi, N.N. Sandal, S.U. Andersen and S. Sato, 2018. Lotus japonicus genetic, mutant and germplasm resources. Curr. Protocols Plant Biol., Vol. 3, No. 2. 10.1002/cppb.20070.
    CrossRef  |  Direct Link  |  
  3. Wee, C.D., M. Hashiguchi, T. Anai, A. Suzuki and R. Akashi, 2017. Fatty acid composition and distribution in wild soybean (Glycine soja) seeds collected in Japan. Asian J. Plant Sci., 16: 52-64.
    CrossRef  |  Direct Link  |  
  4. Nitthaisong, P., G. Ishigaki, K. Fukuyama and R. Akashi, 2017. Evaluation of agronomic traits of some Brachiaria cultivars in the temperate low warm region in Japan. Grassland Sci., 63: 79-84.
    CrossRef  |  Direct Link  |  
  5. Hashiguchi, M., R. Puspasari, Y. Suematsu, M. Muguerza and H. Tanaka et al., 2017. Induction of tetraploid Lotus japonicus and interspecific hybridization with super-root-derived Lotus corniculatus regenerants. Crop Sci., 57: 2387-2394.
    CrossRef  |  Direct Link  |  
  6. Gondo, T., N. Umami, M. Muguerza and R. Akashi, 2017. Plant regeneration from embryogenic callus derived from shoot apices and production of transgenic plants by particle inflow gun in dwarf napier grass (Pennisetum purpureum Schumach.). Plant Biotechnol., 34: 143-150.
    CrossRef  |  Direct Link  |  
  7. Asanuma, Y., T. Gondo, G. Ishigaki, K. Inoue, N. Zaita, M. Muguerza and R. Akashi, 2017. Field trial of insect-resistant and herbicide-tolerant genetically modified cotton (Gossypium hirsutum L.) for environmental risk assessment in Japan. GM Crops Food, 8: 106-116.
    CrossRef  |  Direct Link  |  
  8. Yamamoto, A., M. Hashiguchi, R. Akune, T. Masumoto, M. Muguerza, Y. Saeki and R. Akashi, 2016. The relationship between salt gland density and sodium accumulation/secretion in a wide selection from three Zoysia species. Aust. J. Bot., 64: 277-284.
    CrossRef  |  Direct Link  |  
  9. Tanaka, H., R. Tokunaga, M. Muguerza, Y. Kitazaki and M. Hashiguchi et al., 2016. Genetic structure and speciation of zoysiagrass ecotypes collected in Japan. Crop Sci., 56: 818-826.
    CrossRef  |  Direct Link  |  
  10. Tanaka, H., H. Hirakawa, S. Kosugi, S. Nakayama and A. Ono et al., 2016. Sequencing and comparative analyses of the genomes of zoysiagrasses. DNA Res., 23: 171-180.
    CrossRef  |  Direct Link  |  
  11. Tanaka, H., H. Hirakawa, M. Muguerza, M. Hashiguchi, S. Tabata, R. Akashi and S. Sato, 2016. The complete chloroplast genome sequence of Zoysia matrella (L.) Merr. Crop Sci., 56: 1206-1212.
    CrossRef  |  Direct Link  |  
  12. Tanaka, H., A. Chotekajorn, S. Kai, G. Ishigaki, M. Hashiguchi and R. Akashi, 2016. Determination of genome size, chromosome number and genetic variation using inter-simple sequence repeat markers in Lotus spp. Cytologia, 81: 95-102.
    CrossRef  |  Direct Link  |  
  13. Nitthaisong, P., G. Ishigaki, H. Tanaka and R. Akashi, 2016. Chromosome number, genomic variation and molecular markers to assess genetic diversity of Brachiaria species. Crop Sci., 56: 312-321.
    CrossRef  |  Direct Link  |  
  14. Nakamura, K., K. Kondo, H. Akiyama, T. Ishigaki and A. Noguchi et al., 2016. Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method. Food Chem., 205: 272-279.
    CrossRef  |  Direct Link  |  
  15. Nakamura, K., K. Kondo, H. Akiyama, T. Ishigaki and A. Noguchi et al., 2016. Interlaboratory validation data on real-time polymerase chain reaction detection for unauthorized genetically modified papaya line PRSV-YK. Data Brief, 7: 1165-1170.
    CrossRef  |  Direct Link  |  
  16. Monda, K., H. Araki, S. Kuhara, G. Ishigaki and R. Akashi et al., 2016. Enhanced stomatal conductance by a spontaneous Arabidopsis tetraploid, Me-0, results from increased stomatal size and greater stomatal aperture. Plant Physiol., 170: 1435-1444.
    CrossRef  |  Direct Link  |  
  17. Rahman, M.M., M.R. Rahman, T. Nakagawa, R.B. Abdullah, W.W. Khadijah and R. Akashi, 2015. Effects of wet soya waste supplementation on the intake, growth and reproduction of goats fed Napier grass. Anim. Feed Sci. Technol., 199: 104-112.
    CrossRef  |  Direct Link  |  
  18. Nagata, M., N. Yamamoto, T. Shigeyama, Y. Terasawa and T. Anai et al., 2015. Red/far red light controls arbuscular mycorrhizal colonization via jasmonic acid and strigolactone signaling. Plant Cell Physiol., 56: 2100-2109.
    CrossRef  |  Direct Link  |  
  19. Yano, T., H. Tanaka, T. Kurino, A. Yamamoto, H. Kunitake, Y. Saeki and R. Akashi, 2014. Functional genetic analysis of Arabidopsis thaliana SYNC1 in Lotus corniculatus super-growing roots using the FOX gene-hunting system. Plant Root, 9: 6-14.
    CrossRef  |  Direct Link  |  
  20. Yano, T., A. Yamamoto, H. Kunitake, Y. Saeki and R. Akashi, 2014. The phenomenon of root elongation and high respiration activity in the rolB-gene-enhanced FSL#35 variant of Lotus corniculatus FOX-SR line. Plant Root, 8: 82-91.
    CrossRef  |  Direct Link  |  
  21. Muguerza, M., T. Gondo, G. Ishigaki and R. Akashi, 2014. Lignin content and digestibility in transgenic Bahiagrass (Paspalum notatum Flügge) obtained by genetic manipulation of cinnamyl alcohol dehydrogenase gene. Asian J. Plant Sci., 13: 8-17.
    CrossRef  |  Direct Link  |  
  22. Ishigaki, G., T. Gondo, M.M. Rahman, N. Umami and R. Akashi, 2014. Spontaneous appearance of polyploids in plants regenerated from embryogenic calli derived from seedling‐meristems of ruzigrass (Brachiaria ruziziensis Germain et Everard). Grassland Sci., 60: 24-30.
    CrossRef  |  Direct Link  |  
  23. Himuro, Y., K. Ishiyama, F. Mori, T. Gondo and F. Takahashi et al., 2014. Arabidopsis galactinol synthase AtGolS2 improves drought tolerance in the monocot model Brachypodium distachyon. J. Plant Physiol., 171: 1127-1131.
    CrossRef  |  Direct Link  |  
  24. Rahman, M.M., R.B. Abdullah, W.K.W. Embong, T. Nakagawa and R. Akashi, 2013. Effect of palm kernel cake as protein source in a concentrate diet on intake, digestibility and live weight gain of goats fed Napier grass. Trop. Anim. Health Prod., 45: 873-878.
    CrossRef  |  Direct Link  |  
  25. Rahman, M.M., R.B. Abdullah, W.E.W. Khadijah, T. Nakagawa and R. Akashi, 2013. Feed intake, digestibility and growth performance of goats offered Napier grass supplemented with molasses protected palm kernel cake and soya waste. Asian J. Anim. Vet. Adv., 8: 527-534.
    CrossRef  |  
  26. Muguerza, M., T. Gondo, M. Yoshida, A. Kawakami, F. Terami, T. Yamada and R. Akashi, 2013. Modification of the total soluble sugar content of the C4 grass Paspalum notatum expressing the wheat-derived sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase genes. Grassland Sci., 59: 196-204.
    CrossRef  |  Direct Link  |  
  27. Hashiguchi, T., Y. Sakakibara, Y. Hara, T. Shimohira and K. Kurogi et al., 2013. Identification and characterization of a novel kaempferol sulfotransferase from Arabidopsis thaliana. Biochem. Biophys. Res. Commun., 434: 829-835.
    CrossRef  |  Direct Link  |  
  28. Umami, N., T. Gondo, H. Tanaka, M.M. Rahman and R. Akashi, 2012. Efficient nursery plant production of dwarf cogongrass (Imperata cylindrica L.) through mass propagation in liquid culture. Grassland Sci., 58: 201-207.
    CrossRef  |  Direct Link  |  
  29. Umami, N., T. Gondo, G. Ishigaki, M.M. Rahman and R. Akashi, 2012. Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.). Nihon Danchi Chikusan Gakkaihou, 55: 121-127.
    CrossRef  |  Direct Link  |  
  30. Tominaga, A., T. Gondo, R. Akashi, S.H. Zheng, S. Arima and A. Suzuki, 2012. Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus japonicus. J. Plant Res., 125: 395-406.
    CrossRef  |  Direct Link  |  
  31. Shigeyama, T., A. Tominaga, S. Arima, T. Sakai and S. Inada et al., 2012. Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant. Plant Signal. Behav., 7: 746-748.
    CrossRef  |  Direct Link  |  
  32. Ishigaki, G., T. Gondo, K. Suenaga and R. Akashi, 2012. Fertile transgenic Brachiaria ruziziensis (ruzigrass) plants by particle bombardment of tetraploidized callus. J. Plant Physiol., 169: 546-549.
    CrossRef  |  Direct Link  |  
  33. Akashi, R., N.N. Fancy, A.M. Tanmoy and H. Khan, 2012. Estimation of genome size of jute (Corchorus capsularis (L.) var. CVL-1 using flow cytometry. Plant Tissue Cult. Biotechnol., 22: 83-86.
    Direct Link  |  
  34. Suzuki, A., L. Suriyagoda, T. Shigeyama, A. Tominaga and M. Sasaki et al., 2011. Lotus japonicus nodulation is photomorphogenetically controlled by sensing the Red/Far Red (R/FR) ratio through Jasmonic Acid (JA) signaling. Proc. Natl. Acad. Sci. USA., 108: 16837-16842.
    CrossRef  |  Direct Link  |  
  35. Nishiwaki, A., A. Mizuguti, S. Kuwabara, Y. Toma and G. Ishigaki et al., 2011. Discovery of natural Miscanthus (Poaceae) triploid plants in sympatric populations of Miscanthus sacchariflorus and Miscanthus sinensis in Southern Japan. Am. J. Bot., 98: 154-159.
    CrossRef  |  Direct Link  |  
  36. Himuro, Y., H. Tanaka, M. Hashiguchi, T. Ichikawa and M. Nakazawa et al., 2011. FOX-superroots of Lotus corniculatus, overexpressing Arabidopsis full-length cDNA, show stable variations in morphological traits. J. Plant Physiol., 168: 181-187.
    CrossRef  |  Direct Link  |  
  37. Hashiguchi, M., S.I. Tsuruta and R. Akashi, 2011. Morphological traits of Lotus japonicus (Regal) ecotypes collected in Japan. Interdisciplin. Bio Central, 3: 1-7.
    CrossRef  |  Direct Link  |  
  38. Hashiguchi, M., J. Abe, T. Aoki, T. Anai, A. Suzuki and R. Akashi, 2011. The National BioResource Project (NBRP) Lotus and Glycine in Japan. Breed. Sci., 61: 453-461.
    CrossRef  |  Direct Link  |  
  39. Asanuma, Y., T. Jinkawa, H. Tanaka, T. Gondo, N. Zaita and R. Akashi, 2011. Assays of the production of harmful substances by genetically modified oilseed rape (Brassica napus L.) plants in accordance with regulations for evaluating the impact on biodiversity in Japan. Transgenic Res., 20: 91-97.
    CrossRef  |  Direct Link  |  
  40. Tsuruta, S.I., M. Ebina, M. Kobayashi, R. Akashi and O. Kawamura, 2010. Structure and expression profile of the cinnamyl alcohol dehydrogenase gene and its association with lignification in the sorghum (Sorghum bicolor (L.) Moench) bmr-6 mutant. Breed. Sci., 60: 314-323.
    CrossRef  |  Direct Link  |  
  41. Tominaga, A., M. Nagata, K. Futsuki, H. Abe and T. Uchiumi et al., 2010. Effect of abscisic acid on symbiotic nitrogen fixation activity in the root nodules of Lotus japonicus. Plant Signal. Behav., 5: 440-443.
    CrossRef  |  Direct Link  |  
  42. Okazaki, S., S. Okabe, M. Higashi, Y. Shimoda and S. Sato et al., 2010. Identification and functional analysis of type III effector proteins in Mesorhizobium loti. Mol. Plant-Microbe Interact., 23: 223-234.
    CrossRef  |  Direct Link  |  
  43. Nakamura, K., T. Sugita, H. Tanaka and R. Akashi, 2010. Genetic relationship among sweetpea cultivars and related species by AFLP analysis. J. Jpn. Soc. Hortic. Sci., 79: 360-366.
    CrossRef  |  Direct Link  |  
  44. Nakamura, K., K. Fukumoto and R. Akashi, 2010. Genetic variability of morphological and cultural characteristics in sweetpea (Lathyrus odoratus L.). J. Jpn. Soc. Hortic. Sci., 79: 179-191.
    CrossRef  |  Direct Link  |  
  45. Matsuyama, T., S. Idota, N. Horomoto, T. Otabara, Y. Nomura, H. Ssamoto and R. Akashi, 2010. Effect of growth rate and sprout promotion on the bivalent iron ions in Japanese lawn grass (Zoysia japonica Steud.). J. Jpn. Soc. Turfgrass Sci., 38: 162-166.
  46. Kurata, N., H. Satoh, H. Kitano, Y. Nagato and T. Endo et al., 2010. NBRP, National Bioresource Project of Japan and plant bioresource management. Breed. Sci., 60: 461-468.
    CrossRef  |  Direct Link  |  
  47. Kai, S., H. Tanaka, M. Hashiguchi, H. Iwata and R. Akashi, 2010. Analysis of genetic diversity and morphological traits of Japanese Lotus japonicus for establishment of a core collection. Breed. Sci., 60: 436-446.
    CrossRef  |  Direct Link  |  
  48. Ishigaki, G., T. Gondo, M. Ebina, K. Suenaga and R. Akashi, 2010. Estimation of genome size in Brachiaria species. Grassland Sci., 56: 240-242.
    CrossRef  |  Direct Link  |  
  49. Himuro, Y., H. Tanaka, M. Hashiguchi, R. Yamashige, Y. Shimajiri and R. Akashi, 2010. Characteristics of FSL-PIP-#4 as overexpressed by Arabidopsis thaliana plasma membrane aquaporin gene, AtPIP1;2. Root Res., 19: 143-149.
  50. Tominaga, A., M. Nagata, K. Futsuki, H. Abe and T. Uchiumi et al., 2009. Enhanced nodulation and nitrogen fixation in the abscisic acid low-sensitive mutant enhanced nitrogen fixation1 of Lotus japonicus. Plant Physiol., 151: 1965-1976.
    CrossRef  |  Direct Link  |  
  51. Tanaka, H., J. Toyama and R. Akashi, 2009. Molecular characterization of a galactose-binding lectin from Momordica charantia seeds and its expression in tobacco cells. Asian J. Plant Sci., 8: 544-550.
    CrossRef  |  Direct Link  |  
  52. Shigeki, Y., Y. Kutsuna, K. Yamamoto, H. Isobe, T. Abe, M. Hashiguchi and R. Akashi, 2009. Establishment of multiple-shoot formation from apical meristems and dwarf strain induced by heavy ion beam irradiation in cogongrass (Imperata cylindrica L.). Jpn. J. Grassland Sci., 55: 227-232.
  53. Ishigaki, G., T. Gondo, K. Suenaga and R. Akashi, 2009. Multiple shoot formation, somatic embryogenesis and plant regeneration from seed-derived shoot apical meristems in ruzigrass (Brachiaria ruziziensis). Grassland Sci., 55: 46-51.
    CrossRef  |  Direct Link  |  
  54. Ishigaki, G., T. Gondo, K. Suenaga and R. Akashi, 2009. Induction of tetraploid ruzigrass (Brachiaria ruziziensis) plants by colchicine treatment of in vitro multiple‐shoot clumps and seedlings. Grassland Sci., 55: 164-170.
    CrossRef  |  Direct Link  |  
  55. Himuro, Y., T. Gondo, K. Yamakawa and R. Akashi, 2009. Genetic transformation of bahiagrass (Paspalum notatum Flügge) by visually screening cells expressing green fluorescent protein. Grassland Sci., 55: 216-220.
    CrossRef  |  Direct Link  |  
  56. Gondo, T., J. Matsumoto, S.I. Tsuruta, M. Yoshida and A. Kawakami et al., 2009. Particle inflow gun-mediated transformation of multiple-shoot clumps in rhodes grass (Chloris gayana). J. Plant Physiol., 166: 435-441.
    CrossRef  |  Direct Link  |  
  57. Umezawa, T., T. Sakurai, Y. Totoki, A. Toyoda and M. Seki et al., 2008. Sequencing and analysis of approximately 40 000 soybean cDNA clones from a full-length-enriched cDNA library. DNA Res., 15: 333-346.
    CrossRef  |  Direct Link  |  
  58. Tsuruta, S.I., F. Hosaka, T. Otabara, M. Hashiguchi, T. Yamamoto and R. Akashi, 2008. Genetic diversity of chloroplast DNA in Zoysia and other warm-season turfgrasses. Grassland Sci., 54: 151-159.
    CrossRef  |  Direct Link  |  
  59. Toyama, J., H. Tanaka, A. Horie, T. Uchiyama and R. Akashi, 2008. Purification and characterization of Anti-H lectin from the seed of Momordica charantia and the inter-specific differences of hemagglutinating activity in Cucurbitaceae. Asian J. Plant Sci., 7: 647-653.
    CrossRef  |  Direct Link  |  
  60. Tanaka, H., J. Toyama, M. Hashiguchi, Y. Kutsuna, S.I. Tsuruta, R. Akashi and F. Hoffmann, 2008. Transgenic superroots of Lotus corniculatus can be regenerated from superroot-derived leaves following Agrobacterium-mediated transformation. J. Plant Physiol., 165: 1313-1316.
    CrossRef  |  Direct Link  |  
  61. Nakamura, K., H. Hino, S. Gunji, N. Hattanda and T. Murata et al., 2008. 'Mimi' sweet pea for forcing culture. HortScience, 43: 2238-2239.
    CrossRef  |  Direct Link  |  
  62. Morishita, F., S. Idota and R. Akashi, 2008. Effects of peptized silica application on growth and mineral content in creeping bentgrass. Bull. Fac. Agric. Miyazaki Univ., 54: 65-68.
  63. Huang, L., A. Ikejiri, Y. Shimizu, T. Adachi and Y. Goto et al., 2008. Immunoadjuvant activity of crude lectin extracted from Momordica charantia seed. J. Vet. Med. Sci., 70: 533-535.
    CrossRef  |  Direct Link  |  
  64. Uchiyama, T., S. Kai, S. Tanimura, K. Sata, O. Kawamura and R. Akashi, 2007. Identification of plant species through the detection of the cell wall components using pyrolysis-gas chromatography. Jpn. J. Sci. Technol. Identif., 12: 185-196.
  65. Tsuruta, S.I., M. Ebina, H. Nakagawa, O. Kawamura and R. Akashi, 2007. Isolation and characterization of cDNA encoding Cinnamyl Alcohol Dehydrogenase (CAD) in sorghum (Sorghum bicolor (L.) Moench). Grassland Sci., 53: 103-109.
    CrossRef  |  Direct Link  |  
  66. Hashiguchi, M., S. Tsuruta, T. Matsuo, M. Ebina, M. Kobayashi, H. Akamine and R. Akashi, 2007. Analysis of genetic resource in Zoysia spp. 2: Evaluation of genetic diversity in zoysiagrass indigenous to Southwest islands of Japan based on simple sequence repeat markers. Jpn. J. Grassland Sci., 53: 133-137.
  67. Gondo, T., S. Sato, K. Okumura, S. Tabata, R. Akashi and S. Isobe, 2007. Quantitative trait locus analysis of multiple agronomic traits in the model legume Lotus japonicus. Genome, 50: 627-637.
    CrossRef  |  Direct Link  |  
  68. Gondo, T., J. Matsumoto, K. Yamakawa, S.I. Tsuruta, M. Ebina and R. Akashi, 2007. Somatic embryogenesis and multiple-shoot formation from seed-derived shoot apical meristems of rhodesgrass (Chloris gayana Kunth). Grassland Sci., 53: 138-142.
    CrossRef  |  Direct Link  |  
  69. Ebina, M., K. Kouki, S.I. Tsuruta, R. Akashi and T. Yamamoto et al., 2007. Genetic relationship estimation in guineagrass (Panicum maximum Jacq.) assessed on the basis of simple sequence repeat markers. Grassland Sci., 53: 155-164.
    CrossRef  |  Direct Link  |  
  70. Uciyama, T., R. Hisazumi, I. Maeda, M. Nakata and R. Ohno et al., 2006. DNA extraction from sperm found in mixed stains of seminal fluid and vaginal secretion using DNase I. Jpn. J. Sci. Technol. Identif., 11: 105-112.
  71. Shimizu, T., M. Toyosato, Y. Kutsuna, Y. Saeki and K. Minamizawa et al., 2006. Use of green fluorescent protein to trace the N2-fixing endophytic bacterium Herbaspirillum inoculated in a sugarcane plant. Soil Microorg., 60: 3-9.
  72. Saleng, K., A. Sawai, T. Nagatani, Y. Tateyama and N. Mochidome et al., 2006. Dry matter production and in vitro digestibility in BC1 progeny of bahiagrass (Paspalum notatum Flugge) regenerated from suspension culture. Grassland Sci., 52: 81-84.
    CrossRef  |  Direct Link  |  
  73. Hashiguchi, M., S. Tsuruta, T. Matsuo, H. Akamine and R. Akashi, 2006. Analysis of genetic resource in Zoysia spp. 1: Morphological characteristics and covering gain in zoysiagrass indigenous to Southwest Islands of Japan. Jpn. J. Grassland Sci., 52: 183-189.
  74. Tsuruta, S.I., M. Hashiguchi, M. Ebina, T. Matsuo and T. Yamamoto et al., 2005. Development and characterization of simple sequence repeat markers in Zoysia japonica Steud. Grassland Sci., 51: 249-257.
    CrossRef  |  Direct Link  |  
  75. Gondo, T., S.I. Tsuruta, R. Akashi, O. Kawamura and F. Hoffmann, 2005. Green, herbicide-resistant plants by particle inflow gun-mediated gene transfer to diploid bahiagrass (Paspalum notatum). J. Plant Physiol., 162: 1367-1375.
    CrossRef  |  Direct Link  |  
  76. Oguchi, K., A. Inoue, R. Akashi, Y. Saeki, Y. Nagatomo, S Akao and H. Takaki, 2004. Effect of zinc on root formation in “Super-growing root” of bird's-foot trefoil (Lotus corniculatus L.). Jpn. J. Soil Sci. Plant Nutr., 75: 9-14.
  77. Nasrullah, M. Niimi, R. Akashi and O. Kawamura, 2004. Nutritive evaluation of forage plants grown in South Sulawesi, Indonesia II. Mineral composition. Asian-Australas. J. Anim. Sci., 17: 63-67.
    CrossRef  |  Direct Link  |  
  78. Gondo, T., R. Akashi and O. Kawamura, 2004. Effect of BAP and cupric sulfate on embryogenic callus formation and plant regeneration in bahiagrass (Paspalum notatum Flügge). Jpn. J. Grassland Sci., 50: 55-58.
  79. Tsuruta, S., Y. Sakatani, T. Kawabe, T. Kawano, M. Ebina, R. Akashi and O. Kawamura, 2003. Enzymatic activities on the phenylpropanoid metabolism and their relationships with an accumulation of lignin in brown-midrib-6 (bmr-6) mutation of sorghum (Sorghum bicolor L. Moench). Jpn. J. Grassland Sci., 49: 379-383.
  80. Saleng, K., M. Nasu, M. Niimi, R. Akashi and O. Kawamura, 2003. Variations in morphological and feeding characteristics in plants regenerated from embryogenic suspension cultures derived from a single genotype of bahiagrass (Paspalum notatum Flugge). Jpn. J. Grassland Sci., 49: 456-459.
    CrossRef  |  Direct Link  |  
  81. Nasrullah, M.N., R. Akashi and O. Kawamura, 2003. Nutritive evaluation of forage plants grown in South Sulawesi, Indonesia. Asian-Aust. J. Anim. Sci., 16: 693-701.
    Direct Link  |  
  82. Gondo, T., Y. Ishii, R. Akashi and O. Kawamura, 2003. Efficient embryogenic callus induction derived from mature seed and the examination of the genetic transformation condition by particle bomberdment in bahiagrass (Paspalum notatum Flügge). Jpn. J. Grassland Sci., 49: 33-37.
  83. Akashi, R., T. Kawano, M. Hashiguchi, Y. Kutsuna, S.S. Hoffmann-Tsay and F. Hoffmann, 2003. Super roots in Lotus corniculatus: A unique tissue culture and regeneration system in a legume species. Plant Soil, 255: 27-33.
    CrossRef  |  Direct Link  |  
  84. Akashi, R., T. Gondo and O. Kawamura, 2003. Fundamental studies on the improvement of some warm-season grasses by means of plant biotechnology. Grassland Sci., 49: 79-87 (In Japanese).
  85. Tsuruta, S., R. Akashi, O. Kawamura and H. Numaguchi, 2002. Phylogenetic variation of morphological characteristics and their relationship with feed compositions in napiagrass (Pennisetum purpureum Schumach.). Jpn. J. Grassland Sci., 47: 604-609.
  86. Tsuruta, S., R. Akashi, O. Kawamura and H. Numaguchi, 2002. Molecular phylogenetic relationships among fifteen lines of Napier grass (Pennisetum purpureum Schumach.) using RAPD analysis. Jpn. J. Grassland Sci., 48: 37-42.
  87. Kawamura, O., N. Niijima, M. Niimi and R. Akashi, 2002. Dry matter and lignin distributions determined after micro-surgical tissue separation in stem of maize (Zea mays L.) at different maturation stages. Grassland Sci., 47: 578-582.
  88. Akashi, R., Y. Chie, T. Gondo, O. Kawamura and F. Hoffmann, 2002. Bialaphos-resistant cells of dallisgrass (Paspalum dilatatum Poir.) through particle bombardment with a simple self-built inflow gun. Grassland Sci., 47: 588-593.
  89. Akashi, R., S. Harris, S.S. Hoffmann-Tsay and F. Hoffmann, 2000. Plants from protoplasts isolated from a long-term root culture (Super Root) of Lotus corniculatus. J. Plant Physiol., 157: 215-221.
    CrossRef  |  Direct Link  |  
  90. Akashi, R., T. Uchiyama, A. Sakamoto, O. Kawamura and F. Hoffmann, 1998. High-frequency embryogenesis from cotyledons of bird's-foot trefoil (Lotus corniculatus) and its effective utilization in Agrobacterium tumefaciens-mediated transformation. J. Plant Physiol., 152: 84-91.
    CrossRef  |  Direct Link  |  
  91. Akashi, R., S.S. Hoffmann-Tsay and F. Hoffmann, 1998. Selection of a super-growing legume root culture that permits controlled switching between root cloning and direct embryogenesis. Theoret. Applied Genet., 96: 758-764.
    CrossRef  |  Direct Link  |  
  92. Akashi, R. and O. Kawamura, 1998. Improvement of forage quality in Bahiagrass (Paspalum notatum Flugge) through genetic manipulation.: 1. Detection of somaclonal variation using RAPD analysis in plants regenerated from suspension cultures. Jpn. J. Grassland Sci., 44: 203-207.
    CrossRef  |  Direct Link  |  
  93. Hossain, B., N. Hiraata, Y. Nagatomo, R. Akashi and H. Takaki, 1997. Internal zinc accumulation is correlated with increased growth in rice suspension culture. J. Plant Growth Regul., 16: 239-243.
    CrossRef  |  
  94. Akashi, R., S. Lachmann, F. Hoffmann and T. Adachi, 1995. Embryogenic callus formation from protoplasts derived from suspension cells of apomictic guineagrass (Panicum maximum Jacq.). Jpn. J. Breed., 45: 445-448.
    CrossRef  |  Direct Link  |  
  95. Akashi, R., A. Hashimoto and T. Adachi, 1993. Plant regeneration from seed-derived embryogenic callus and cell suspension cultures of bahiagrass (Paspalum notatum). Plant Sci., 90: 73-80.
    CrossRef  |  Direct Link  |  
  96. Akashi, R. and T. Adachi, 1992. Somatic embryogenesis and plant regeneration from cultured immature inflorescences of apomictic dallisgrass (Paspalum dilatatum Poir.). Plant Sci., 82: 213-218.
    CrossRef  |  Direct Link  |  
  97. Akashi, R. and T. Adachi, 1992. Plant regeneration from suspension cultured-derived protoplasts of apomictic dallisgrass (Paspalum dilatatum Poir.). Plant Sci., 82: 219-225.
    CrossRef  |  Direct Link  |  
  98. Kishima, Y., K. Nozaki, R. Akashi and T. Adachi, 1991. Light-inducible pigmentation in Portulaca callus; selection of a high betalain producing cell line. Plant Cell Rep., 10: 304-307.
    CrossRef  |  Direct Link  |  
  99. Akashi, R. and T. Adachi, 1991. High frequency somatic embryo formation in cultures of immature embryos of guineagrass, Panicum maximum Jacq. Jpn. J. Breed., 41: 85-93.
    CrossRef  |  Direct Link  |  
  100. Akashi, R. and H. Ikeda, 1989. Callus formation and plant regeneration in some wild legume species. Plant Tissue Cult. Lett., 6: 31-32.
    CrossRef  |  Direct Link  |  
  101. Akashi, R. and H. Ikeda, 1989. Callus formation and plant regeneration from immature inflorescences and apical meristems of cogongrass (Imperata cylindrica L.). Jpn. J. Grassland Sci., 34: 333-335.
    CrossRef  |  Direct Link  |  
  102. Akashi, R. and H. Ikeda, 1987. Callus formation and plant regeneration from immature inflorescences of timothy (Phleum pratense L.). Jpn. J. Grassland Sci., 33: 291-292.